Journal: Journal of Radiation Research

Article Title: YC-1 sensitizes the antitumor effects of boron neutron capture therapy in hypoxic tumor cells

doi: 10.1093/jrr/rraa024

Figure Lengend Snippet: Study protocol. Cells were seeded and incubated for 12 h in ( A ) 21% O 2 (normal oxygen conditions) or 1% O 2 (hypoxic conditions), and ( B ) normal oxygen conditions with or without DFO for 24 h. In both time courses, after incubation for 24 h, total RNA was extracted and first-strand cDNA was synthesized. LAT1 expression was assessed by qRT-PCR. In the time course in (A), BPA was added to the culture medium with short-term interruption of the hypoxic condition, and then returned to the previous condition. After treatment with BPA for 2 h, boron accumulation was measured by inductively coupled plasma atomic emission spectroscopy. To evaluate the surviving fraction, after treatment with BPA for 2 h, cells were exposed to neutron beams. After neutron irradiation, cells were resuspended by trypsinization, plated onto dishes, cultured for 10-13 days and then used for the clonogenic assay.

Article Snippet: 10 B-Enriched L-BPA solution and yttrium inductively coupled plasma standard solution were kindly supplied by Stella Pharma Corporation (Osaka, Japan).

Techniques: Incubation, Synthesized, Expressing, Quantitative RT-PCR, Clinical Proteomics, Spectroscopy, Irradiation, Cell Culture, Clonogenic Assay

Journal: Toxins

Article Title: A Multi-Layer-Controlled Strategy for Cloning and Expression of Toxin Genes in Escherichia coli

doi: 10.3390/toxins15080508

Figure Lengend Snippet: Spot test of serial diluted toxins on plates with or without inducing agents for the P fdeA —RS B12 strategy. E. coli EPI400 harboring the successfully cloned toxins P1 Doc, P1 Doc H66Y , Ec MazF, Ec MazF E24A , Ec ParE2, Vc HigB2, barnase and FCcdB on the pJYP1 vector was spotted on LB agar plates supplemented with ampicillin and additionally vitamin B 12 ( A , OFF-state) or naringenin ( B , ON-state) or a combination of both ( C , partly induced) or none of the additional agents ( D , partly induced).

Article Snippet: This was used to inoculate 10 mL of fresh LB with 100 μg/mL of ampicillin, 1× CopyCutter Induction Solution (LGC Biosearch Technologies, Teddington, England) and 50 nM vitamin B 12 , which was added to reach an OD 600 of 0.2.

Techniques: Spot Test, Clone Assay, Plasmid Preparation

Journal: Toxins

Article Title: A Multi-Layer-Controlled Strategy for Cloning and Expression of Toxin Genes in Escherichia coli

doi: 10.3390/toxins15080508

Figure Lengend Snippet: Logarithmic normalized overview of cell growth observed for the P fdeA -RS B12 strategy. Colonies are counted from the spot test on different LB ampicillin plates for E. coli EPI400 harboring toxins P1 Doc, P1 Doc H66Y , Ec MazF, Ec MazF E24A , Ec ParE2, Vc HigB2, barnase and F CcdB. Raw colony count data were normalized to fraction survival for comparison of the OFF-state (vitamin B 12 and no naringenin, black bars) to the ON-state (naringenin and no vitamin B 12 , grey bars).

Article Snippet: This was used to inoculate 10 mL of fresh LB with 100 μg/mL of ampicillin, 1× CopyCutter Induction Solution (LGC Biosearch Technologies, Teddington, England) and 50 nM vitamin B 12 , which was added to reach an OD 600 of 0.2.

Techniques: Spot Test, Comparison

Journal: Toxins

Article Title: A Multi-Layer-Controlled Strategy for Cloning and Expression of Toxin Genes in Escherichia coli

doi: 10.3390/toxins15080508

Figure Lengend Snippet: Spot test of serial diluted toxins on plates with or without inducing agents for the P tac —RS theo strategy. E. coli EPI400 harboring the successfully cloned toxins P1 Doc*, P1 Doc H66Y , Ec MazF, Ec MazF E24A , Ec ParE2, Vc HigB2, barnase* and F CcdB* on the pJYP3 vector were spotted on LB agar plates supplemented with ampicillin ( A , OFF-state) and additionally theophylline and IPTG ( B , ON-state) or IPTG alone ( C , partly induced) or theophylline alone ( D , partly induced).

Article Snippet: This was used to inoculate 10 mL of fresh LB with 100 μg/mL of ampicillin, 1× CopyCutter Induction Solution (LGC Biosearch Technologies, Teddington, England) and 50 nM vitamin B 12 , which was added to reach an OD 600 of 0.2.

Techniques: Spot Test, Clone Assay, Plasmid Preparation

Journal: Toxins

Article Title: A Multi-Layer-Controlled Strategy for Cloning and Expression of Toxin Genes in Escherichia coli

doi: 10.3390/toxins15080508

Figure Lengend Snippet: Logarithmic normalized overview of cell growth observed for the P tac —RS theo strategy. Colonies are counted from the spot test on different LB ampicillin plates for E. coli EPI400 harboring toxins P1 Doc, P1 Doc H66Y , Ec MazF, Ec MazF E24A , Ec ParE2, Vc HigB2, barnase and F CcdB. Raw colony count data were normalized to fraction survival for comparison of the OFF-state (no theophylline and no IPTG, black bars) to the ON-state (theophylline and IPTG, grey bars).

Article Snippet: This was used to inoculate 10 mL of fresh LB with 100 μg/mL of ampicillin, 1× CopyCutter Induction Solution (LGC Biosearch Technologies, Teddington, England) and 50 nM vitamin B 12 , which was added to reach an OD 600 of 0.2.

Techniques: Spot Test, Comparison

Journal: Toxins

Article Title: A Multi-Layer-Controlled Strategy for Cloning and Expression of Toxin Genes in Escherichia coli

doi: 10.3390/toxins15080508

Figure Lengend Snippet: Spot test of serial diluted E. coli EPI400 harboring pJYP4_ Vc ParE2 on plates with or without inducing agents for the P BAD —RS theo strategy. E. coli EPI400 harboring pJYP4_ Vc ParE2 was spotted on ( A , OFF-state) LB agar plates supplemented with ampicillin and ( B , ON-state) theophylline and arabinose.

Article Snippet: This was used to inoculate 10 mL of fresh LB with 100 μg/mL of ampicillin, 1× CopyCutter Induction Solution (LGC Biosearch Technologies, Teddington, England) and 50 nM vitamin B 12 , which was added to reach an OD 600 of 0.2.

Techniques: Spot Test